From the 2001, IVALSA and Veneto Agricoltura (the agricultural agency of Veneto) are collaborating to the development of in vitro conservation and cryopreservation procedures, suitable for the preservation in safe conditions (genetic and sanitary) of ornamental, vegetable and fruit germplasm of Veneto. The study is carried out at the Experimental Centres “Po di Tramontana” of Rosolina and “Pradon” of Porto Tolle (Rovigo). Effective conservation techniques have been applied to autochthonous varieties of apple and pear, fruit rootstocks, ancient artichoke germplasm, garlic and red chicory typologies, chrysanthemum and rose germplasm. Shoot cultures have been conserved in slow growth storage for 9-12 months, maintaining a very excellent qualitative standard which allows their fast recover of in vitro proliferation. As for cryopreservation, innovative techiques, allowing the direct immersion of specimen in liquid nitrogen, have been applied (vitrification, encapsulation-dehydration, encapsulation-vitrification, droplet freezing). It is presently in progress a study on the cryopreservation of micro-scions from ancient apple accessions of Veneto, to be used for chip-budding on rootstocks. Furthermore, the use of liquid nitrogen for the recovery of virus-free fruit plants (“cryotherapy”) is under investigation. The establishmente of a cryobank of plant germplasm at the “Po di Tramontana” Centre is at present considered; it would be the first example in Italy of application of the cryogenic technology to the preservation of plant biodiversity.