Innovative techniques of micropropagation (= in vitro propagation)

Micropropagation is a plant propagation technique of high importance in Italy. A census of some years ago estimeed in almost 25 millions the plants produced by micropropagation. Fruit plants represent more than 70% of this production, confirming the Italian leadership in this sector in terms of both quantity and quality of micropropagated plants. Although the Italian offer is still not much differentiated (just the peach rootstock ‘GF677’ covers 40% of the whole in vitro production), in recent years the main commercial laboratories      are making an effort to widen the stock of rootstocks and cultivars from not only many fruit species, but also ornamental plants and some vegetables. This is an important signal coming from the sector which will face soon the competition of new commercial laboratories from the East-Europe Countries (Poland, Cech Republic, Slovakia, Greece, Turkey), characterized by a lower cost of labour. This fact imposes to our enterprises to invest in “research and development”, as well as to aim at a high laboratory efficiency, a great diversification of the plant offer, and an excellent quality of plant production. The CNR-IVALSA, with his great expertise in innovative tissue culture techniques, is today an important interlocutor for micropropagation commercial enterprises, proposing and developing qualified experimental activity. Among the various studies carried out at IVALSA as for this sector there are the optimization of specific microproagation protocols in semi-solid media, and the development of innovative tissue culture systems, such as somatic embryogenesis, the production and regeneration of encapsulated explants (synthetic seeds), the liquid culture in temporary immersion system (TIS). The latter technique, in particular, is based on cycles of immersion of shoots in liquid medium, alternated with dry periods of variable length. In comparison with the use of gelled media, the TIS allows a more uniform contact between the colture medium and the shoots, as well as the diluition of toxic compounds (phenols) released from the vegetative material; moreover, the liquid medium can be periodically added to the containers and the accumulation of gases (CO2 and ethylene)  is reduced. A recent study of IVALSA showed that the culture in RITA® (a TIS device) stimulates the development of axillary buds from olive shoots, a species characterized in vitro by a strong apical dominance which allows mainly the elongation of shoots in gelled media. A wide range of fruit, ornamental and forest   species are currently investigated as for in vitro propagation. The developed regeneration systems are also used to optimize effective cryopreservation techniques.  


Maurizio Lambardi (person in charge)
Carla Benelli
Anna De Carlo
Elif Aylin Ozudogru

last publications

Lambardi M., Benelli C., Ozudogru A.E., Ozden-Tokatli Y., 2006. Synthetic seed technology in ornamental plants. In: Teixeira da Silva JA (Ed) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, vol. II. Global Science Books, UK, pp. 347-354.

Lambardi M., Benelli C., Ozden-Tokatli Y., Ozudogru E.A., Gumusel F., 2006. A novel approach to olive micropropagation: the temporary immersion system. Proc. “2nd Int. Seminar OLIVEBIOTEQ 2006”, Vol I. Marsala – Mazara del Vallo, Italy, pp. 319-326.

Ozden-Tokatli Y., De Carlo A., Gumusel F., Pignattelli S., Lambardi M., 2008. Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plants.  Prop. Ornam.. Plants 8(1):13-16.

Previati A., Benelli C., Da Re F., Ozudogru E.A., Lambardi M., 2008. Micropropagation and in vitro conservation of virus-free rose germplasm. Prop. Ornam. Plants 8(2): 93-98.

Amirkhani M., Lambardi M., Mesdaghi M., Mashayekhi K., Ozudogru E.A., 2010. In vitro morphogenesis and plant regeneration from seed- and leaf-derived cali of four Agropyron species. Propagation of Ornamental Plants, 10(2): 99-106.

Lambardi M., Ozudogru A.E., 2010. La propagazione “in vitro” dei portinnesti e delle specie da frutto. Frutticoltura 12: 18-25.

[Last updated on: 02-11-2015]
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